[Yeast] Finding the TF for a short DNA regulatory sequences

Shaoyu Wang via yeast%40net.bio.net (by S.Wang from uws.edu.au)
Sun Sep 20 23:06:07 EST 2009


Dear There,

May I ask a simple help. I have used the RSAT - oligo-analysis tool and
obtained an enriched potential TF binding sequence such as  "CCCCTC" in
the upstream (800) of my list of genes, how would I find the potential
TFs for this sequence. Any tools for this?
 
Thank you in advance

Shaun


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Subject: Yeast Digest, Vol 52, Issue 1

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Today's Topics:

   1. transformation efficiencies in yeast (Francis Smet)


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Message: 1
Date: Wed, 2 Sep 2009 16:37:50 +0200
From: Francis Smet <francis.smet from gmail.com>
Subject: [Yeast] transformation efficiencies in yeast
To: yeast from magpie.bio.indiana.edu
Message-ID:
	<2b5170340909020737y25741d4dnffb8b19c5dcca5a9 from mail.gmail.com>
Content-Type: text/plain; charset="iso-8859-1"

Dear all,

I'm wondering if there is a particular reason why invitrogen stopped
selling the pYD1 yeast surface display vector. An additional query
regarding this matter is if there is a link between the used selection
marker (trp in this case of pYD1) and library transformation
efficiencies. I noticed that newer YSD vectors do not use this selection
marker any more.

many thanks for your comments,

Cheers.
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