[Yeast] 5-FOA

kgeiler77 via yeast%40net.bio.net (by kgeiler from fas.harvard.edu)
Mon Jun 27 12:53:26 EST 2011


Hi,

I was wondering what false positive rate to expect when plating yeast on
5-FOA. Are these false positives due to Ura+ colonies that manage to live on
this media for stochastic reasons (and can be screened for by replica
plating onto 5-FOA a second time), or do they represent mutations that allow
survival of Ura+ colonies on 5-FOA?

Any help, even general comments on false positives and 5-FOA, would be
greatly appreciated.

Thanks,
KG
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