extractin mtDNA from fish scales

[mobo at mc.edu]

First, If you used SDS, it is a strong enzyme inhibitor. I use CTAB, and 
get better results. I have a particularly tough template..ressurection 
fern...that has yet to give me good results. I am going to try some 
column purification techniques, first using a genomic DNA isolation kit 
purchased from Pharmacia (27-5225-01). There was a good DNA extraction 
method in a paper by Hadrys..or Hadrys was an author in Molecular 
Ecology..I think in 1993.