apoptosis detection methods?

[Jane Cox]

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Hi, I've just started using acridine orange stain, which binds to degraded
 DNA and fluoresces in an acidic environment. I seems to work well, but
you  would need a FITC filter for your fluorescent microscope.There is a
protocol  in: Furutani-Seiki et al. Development (1996) 123 229-239  Hope
this helps. Cheers, Jane


Jane Cox, Ph.D.
Dept Pharm. Phys
Saint Louis University Med. School
1402 Sth. Grand Blvd.
St. Louis, MO 63104
(314) 977 6445



----- Original Message -----
From: "Ted Allison" <wallison at umich.edu>
Newsgroups: bionet.organisms.zebrafish
To: <zbrafish at net.bio.net>
Sent: Monday, May 16, 2005 11:44 AM
Subject: apoptosis detection methods?


> Hi -
>
> I am looking for methods to detect apoptosis in zebrafish embryos (other
than TUNEL, Annexin V).
>
> I am curious, for example, if anyone has had success in zebrafish using
antibodies against activated caspases ?
>
> thanks,
> Ted
>
> ---
>
>
>


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