[Zbrafish] Autofluorescence
123
via zbrafish%40net.bio.net
(by gottalovelife from gmail.com)
Tue Apr 1 14:02:42 EST 2008
I'm having issues with autofluorescence interfering with my
fluorescent and confocal imaging. Does anyone know at which
wavelengths the fish will autofluoresce the least? I would like to be
able to do my imaging on 4%PFA fixed fish, but I can do it live as
well. I've already tried sodium borohydride but this does not quench
the autofluorescence adequately.
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