[Zbrafish] Re: UCSF Mission Bay - Guo Lab

[Claudia]

On Apr 9, 3:42 pm, Hadie Khodabakhsh <hadie_k1... from yahoo.com> wrote:
> Good afternoon,
>
> I was wondering if you had any suggestions, or methods that will get zebrafish to mate more. I have already placed my zebrafish in the best environmental conditions, but they just don't seem to mate. So I was wondering if there were any hormonal treatment methods that can be used to influence the zebrafish to mate more?
>
> Thank you,
>
> Hadie Khodabakhsh
>
> (In case you are wondering, I am a research associate at the zebrafish lab of Dr. Guo at UCSF Mission Bay)
>
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Hi Hadie,
try keeping your males and females separate. Do not overfeed -if fish
are too fat they do not reproduce either. We keep all our fish on a
flow through system (to minimize contamination) which helps with
elimination of pheromones too. Make sure your fish are not too old. We
spawn fish that are 3 month to 1.5 years. Of course light cycle... is
also important but you probably doing all that already. If you need to
built a cheap flow though system to hold your broodstock check out my
following article:
*	Hohn, C.M., Petrie-Hanson, L. (2007): Low cost aquatic lab animal
holding system. Zebrafish 4(2):117-122.

Hope this helps,
Claudia