[Zbrafish] Re: blood collection...

[david stachura]

>
what do you mean when you say "survival procedures?"  do you mean  
"how can i keep the blood cells alive?" or "how do i keep the fish  
alive after taking blood?"  they don't have much blood (maybe  
20-50ul), so it's pretty hard to take blood and have the fish survive  
(when they are adults).  however, i am sure it can be done.  i would  
try to do a cardiac puncture with a hamilton syringe.  but i  
guarantee the survival rates are going to be low.  we have tried  
injecting cells into the heart, and maybe kill 1:5 fish.

David Stachura, Ph.D.
Cell and Developmental Biology
University of California at San Diego
9500 Gilman Dr.
Natural Sciences Building 6105
La Jolla, CA 92093-0380
davidstachura from gmail.com
dstachura from ucsd.edu
http://www.biology.ucsd.edu/labs/traver

> ------------------------------
>
> Message: 3
> Date: Thu, 24 Apr 2008 03:43:48 -0700 (PDT)
> From: Doloresbf <dolores.bonaparte from gmail.com>
> Subject: [Zbrafish] survival blood collection?
> To: bionet-organisms-zebrafish from moderators.isc.org
> Message-ID:
> 	<837caaf6-f2a0-4f3c-a88d-3020c87c4490 from c65g2000hsa.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
>
> Are you familiar with survival procedures for blood collection on
> ZFish? Any help would be much appreciated!
> Thanks very much. Best. Dolores
> ................................................
> Dolores Bonaparte, DVM
> Instituto de Medicina Molecular
> Animal Facility
> Av. Prof. Egas Moniz, Edif. Egas Moniz
> 1649-028 Lisboa
> Portugal
>
>
>
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> End of Zbrafish Digest, Vol 35, Issue 15
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