[Zbrafish] GFP and in situ

[Chi-Bin Chien]

Native GFP fluorescence is sensitive to pH and organic solvents, and 
does not survive in situ protocols.

However, in our hands anti-GFP antibody staining after in situs works 
very well. See the following reference:

http://www.jneurosci.org/cgi/content/full/26/51/13328

Chi-Bin Chien

At 1:46 PM -0400 8/1/08, Weibin Zhou wrote:
>Hi,
>I am wondering whether it is possible to preserve GFP fluorescence 
>after in situ hybridization (whole-mount or sectioned). If you 
>happen to have a protocol that works, please let me know. Thank you 
>very much.
>
>Weibin
>
>
>
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