[Zbrafish] Zebrafish Age / Size Variations

[Thomas Bartman]

Oliver,

I think this is an important issue which is sometimes overlooked.  We are looking at late heart development, and are trying to get work published which shows that morphogenesis is tied strongly to body mass (therefore, theoretically physiological parameters) and not age.

There was a poster at the zf meeting which proposed what seemed to be a nice method for staging larvae / young adults based on length and physical characteristics.  I don't have the abstracts in front of me.  Maybe the authors of that work are on this newsgroup and will chime in?

Tom Bartman
Cincinnati Children's Hospital

Thomas Bartman, M.D., Ph.D.
Cincinnati Children's Hospital
Divisions of Neonatology, Pulmonary Biology and Developmental Biology
3333 Burnet Avenue
Cincinnati, OH  45229
(513) 636-9902
>>> Oliver Braubach <braubach from dal.ca> 08/07/08 3:02 PM >>>
Dear All.

I have been working on aspects of postembryonic development in the  
zebrafish brain, and have been trying to determine a standard method  
for 'aging' zebrafish. As many of you may be aware, the development of  
individual fish varies greatly during the first month of development,  
which ultimately leads to very noticeable differences in the size of  
individual fish and their organs.

Thus, age does not accurately characterize developmental stages of  
fish, and I have been trying to determine if there's another standard  
method to do so. Has anyone tried to correlate body length to fish  
age? Are there any citations for expected / optimal size of fish are a  
certain age?

Any input regarding this problem would be greatly appreciated.
Best,

Oliver Braubach
Department of Physiology and Biophysics
Dalhousie University
Halifax, NS B3H 1X5 Canada
Tel: 902.494.6471
Fax: 902.494.1685

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