[Zbrafish] purchased hanks for squeezing?
Jocelyn
via zbrafish%40net.bio.net
(by jocelyn.mcauley from gmail.com)
Tue Aug 12 13:21:37 EST 2008
Has anyone used Gibco's Hanks in an invitro fertilization protocol?
I'm noticing that Gibco's formula includes glucose (to up the
osmolarity?), where as the Zebrafish book recipe does not use glucose.
Will this affect the viability of the sperm?
I need to do a squeeze, and don't have the stock solution components
on hand to make hanks, but instead have the Gibco premade 10x hanks (-
bicarb, - phenol red).
Any advice?
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