[Zbrafish] Re: Bleaching embryos after In Situ Hybridization

[nonolow from gmail.com]

bleaching is a routine approach in frog embryo whole mount in situ
hybridization, i think you could find the protocal and recipes in the
Frog Book (Early Development of Xenopus Laevis: A Laboratory
Manual)....
And be aware the time of bleaching, hydrogen peroxide could breakdown
the staining of in situ hybridization.


low



On Aug 19, 1:22 am, shellyleib... from gmail.com wrote:
> Hello
> I'm Shelly, a PhD student at Gothilf's lab from Tel Aviv University. I
> wanted to ask if anyone knows how to bleach the pigment of 2dpf
> zebrafish embryos post whole mount In Situ Hybridization (it was not
> raised in PTU water).
> Many thanks in advance
>
> Shelly