[Zbrafish] Re: Bleaching embryos after In Situ Hybridization

[nonolow from gmail.com]

bleaching is a routine approach in frog embryos, you could find some
protocols and recipes in the book, Early Development of Xenopus
Laevis: A Laboratory Manual,
be aware the time of bleaching, hydrogen peroxide could breakdown the
staining of in situ hybridization.
good luck


low





On Aug 19, 1:22 am, shellyleib... from gmail.com wrote:


> Hello
> I'm Shelly, a PhD student at Gothilf's lab from Tel Aviv University. I
> wanted to ask if anyone knows how to bleach the pigment of 2dpf
> zebrafish embryos post whole mount In Situ Hybridization (it was not
> raised in PTU water).
> Many thanks in advance
>
> Shelly