[Zbrafish] Help with injected intron containing dsRed mRNA
xiawei
via zbrafish%40net.bio.net
(by xiaw from umbi.umd.edu)
Fri Feb 1 14:09:54 EST 2008
I'm planning to generate some RNAs which was transcribed from pCS2+
plasmid using Ambion mMESSAGEmMACHINE kit and contains a intron
inserted dsRed sequence. Does anybody know if the injected intron
containg RNA can be processed into mature dsRed mRNA and give a
normal expression of red fluorescence?
Thanks very much.
Wei
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