[Zbrafish] Zebrafish: (Big problem) BAC/PAC injection into embryos

[Zhen Fenghua]

Dear all:

I am currently doing a project to screen a transgenic fish line by
injection of PAC/BAC construct into fish embryos. We found a very
interesting phenomenon: the uptake of PAC DNA, or in another words, the
expression of GFP signal is different in different WILD TYPE fish lines,
although all the other conditions are exactly same. Does anyone have such
experiences before?

Anyway, the good news is that we have found a specific wild type fish line
who will carry the best expression of PAC-driven GFP, but the bad news is
that the next generation of this fish line get worse expression of GFP;
therefore, it brings a big trouble to my experiments. So may anyone help
to guess the reason or share any idea with me?

My email address is zhenfh from ust.hk, and I will be very appreciated to any
of your response.

Thanks a lot!

Best regards,
zhen feng hua
Tel: 852-2358-7282
Hong Kong University of Scoence and Technology