[Zbrafish] help with parasite infecting embryos
lampe1
via zbrafish%40net.bio.net
(by lampe1 from umbc.edu)
Thu Jul 31 12:19:05 EST 2008
Hello!
In our zebrafish facility, we frequently have embryos infected with
some sort of parasite. It looks like little filaments of cotton which
surround the embryo. When this happens, the embryos usually die within
24-72 hours. Usually bleaching (according to ZFIN protocol--0.1mL 5%
sodium hypochlorite in 170mL system water, bleach 5 min, system water
5 min, bleach 5 min, rinse 2+ times in system water) and replacing the
water stops this and saves the embryos.
Recently, this parasite has been worse (likely due to our UV
sterilizer being broken for ~1 week). One person in the lab bleached
the embryos and rinsed them several times and yet they still all died
and were infected with this parasite.
Does anyone have any idea what this parasite may be? I am having
trouble since I have no idea what kind of organism it even is. Does
anyone have any ideas for other ways to bleach/otherwise save embryos?
I appreciate any help!
Thanks,
Rebecca Lampe
Fish Care Technician
UMBC Department of Biology
lampe1 from umbc.edu
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