From bdschyth from gmail.com Sat Mar 1 10:50:03 2008 From: bdschyth from gmail.com (bdschyth) Date: Sat Mar 1 12:49:33 2008 Subject: [Zbrafish] Re: help with adult zebrafish organ identification References: Message-ID: <412e986a-c22f-4507-942b-5eeab06a2896@s8g2000prg.googlegroups.com> On Feb 29, 9:31?pm, michelle wrote: > Hello! > > I am a graduate student in the Gastroenterology Department at Boston > University School of Medicine. ?I am currently doing > immunohistochemistry on adult zebrafish sections, and need some help > with organ identification, specifically the intestine and pancreas. > Would anyone be able to look at some pictures and help with > identification? ?Thanks, and I would really appreciate any help or > suggestions that anyone has. > > Michelle Dear Michelle I might be able to help you as we are often looking at organs of rainbow trout. Brian From LiuZijuan from gmail.com Sat Mar 1 16:15:48 2008 From: LiuZijuan from gmail.com (detroitriver) Date: Mon Mar 3 12:09:28 2008 Subject: [Zbrafish] Express genes in ZF-1 cell line Message-ID: <1e3b5a1f-59fe-4d61-a3fb-c34550ec53af@s12g2000prg.googlegroups.com> Hi, I am a new investigator in Oakland University-I am writing to see if anybody know if we can clone genes in vector to transform into ZF cell line? Thank you a lot for sharing! Zijuan From tgenade from gmail.com Mon Mar 3 05:22:07 2008 From: tgenade from gmail.com (Tyrone) Date: Mon Mar 3 12:09:54 2008 Subject: [Zbrafish] Zebrafish PKA Message-ID: <388af0a4-debc-47fd-a552-2b46af8db7b9@i12g2000prf.googlegroups.com> Hello, I am looking for information concerning zebrafish Protein Kinase A. I have found one paper siting its existance but am yet to find a single paper characterising it. Do zebrafish have a PKA enzyme? Does it auto- phosphorylate at teh zebrafish equivalent of Thr197? Has the gene been cloned and sequenced. Is there an NCBI accession number for the DNA sequence or proposed protein sequence? I am not really working with zebrafish at all, but instead Nothobranchius furzeri. I am planning some immunohistochemical work and would like to know what antibodies are available for zebrafish. I'm looking at evolutionary conserved proteins involved in neurological development and so far the antibodies directed at mice have also worked in Xenopus and goldfish... and we assume zebrafish as well. The fish, N. furzeri, develops neurofibrillary tangles with age. These tangles do not occur when the fish are dosed with resveratrol. PKA has been implicated in priming tau for hyperphosphorylation (that results in the neurofibrillary tanlges). We would like to take a look at this. There is a p-CREB antibody that has worked in zebrafish but pCREB is affected by several kinases so its increased nuclear localization would not tell us a great deal. Mammalian PKA autophosphorylates itself at Thr197. This would be better to monitor than pCREB to see if PKA activity is increasing or out of place in aged fish. If anyone can suggest a good reference, NCBI accession number or a source of a phospho-PKA antibody that would be much appreciated! Thanks From caseyroot from yahoo.com Tue Mar 4 13:10:03 2008 From: caseyroot from yahoo.com (casey) Date: Tue Mar 4 13:13:27 2008 Subject: [Zbrafish] Gynogenetic diploid zebrafish Message-ID: I am working on creating gynogenetic diploid zebrafish. I am using The Zebrafish Book as a guide. It has a protocol listed; however, it does not tell you how you know if you've been successful. Does anybody know how to identify gynogenetic diploid embryos? Also, does anybody have a better/different protocol than what is given in The Zebrafish Book? Thanks for your help... From shaukatali134 from gmail.com Tue Mar 4 13:57:28 2008 From: shaukatali134 from gmail.com (shaukat) Date: Tue Mar 4 14:15:40 2008 Subject: [Zbrafish] buffer solution Message-ID: <845eea54-a89f-4cb9-895c-890dd4bf207b@h25g2000hsf.googlegroups.com> I am determining ammonia toxicity on Zebrafish eggs and larvea.I am looking a non lethal buffer solution to maintain pH of ammonia solutions. From austin from rowellbrokaw.com Wed Mar 5 11:05:35 2008 From: austin from rowellbrokaw.com (Austin Bailey) Date: Wed Mar 5 11:35:07 2008 Subject: [Zbrafish] Cabinet Question continued. Message-ID: <353366EF-60BA-4714-91E3-ED2C3709B313@rowellbrokaw.com> Rebecca, I've never heard of phenolic resin breaking down or having any adverse reactions in a laboratory setting. Its really what they're made for. What I'd be more concerned with is the cabinet hardware and detailing which varies from manufacturer to manufacturer. I've seen products out on the market that have plastic casework but fairly cheap steel hinges and drawer tracks. Find out what sort of protection the company is using for these components. They may be powdercoating these components or using higher grade steel. The cabinet is only as good as its weakest component. Sure, the countertop will be the most important. But although these cabinets are intended for laboratory use, most have not been tested under extreme "wet" conditions. And it may not just be filtered tap water. It may be system water with a higher salinity or it could even be brine from the brine shrimp hatchers. Take a look at the drip edges of the countertop. This is especially important if the hardware for the cabinets is less than optimal. If the countertop has no drip (meaning that water can runoff the edge of the counter and wick back directly into the cabinet - possibly reaching the hardware) you may want to talk to the company about adding a drip or have your own facilities people do this for you. It'll keep water either on the counter or the floor and out of the cabinets. Take a look at those items. If you've found a great deal on plastic cabinets, it sounds like a good way to go. Just be aware of the issues above, so you won't be surprised down the line. Austin Austin Bailey Rowell Brokaw Architects, PC 1 East Broadway, Suite 300 Eugene, OR 97401 tel: 541.485.1003 fax:541.485.7344 www.rowellbrokaw.com From busquet.francois from gmail.com Wed Mar 5 03:46:35 2008 From: busquet.francois from gmail.com (=?ISO-8859-1?Q?fran=E7ois?=) Date: Wed Mar 5 11:35:50 2008 Subject: [Zbrafish] Re: buffer solution References: Message-ID: <72be481c-4013-4d14-94ce-3aacbf195741@e31g2000hse.googlegroups.com> On Mar 4, 7:57?pm, shaukat wrote: > I am determining ammonia toxicity on Zebrafish eggs and larvea.I am > looking a non lethal buffer solution to maintain pH of ammonia > solutions. Tris-HCl buffer works for me at 0.1M when dissolved in fish medium; fish medium: Salts composition Concentration [mM] CaCl2 * 2 H2O 2 MgSO4 * 7 H2O 0.5 NaHCO3 0.7 KCl 0.07 hope it can help! francois From mena22787 from excite.com Wed Mar 5 11:36:41 2008 From: mena22787 from excite.com (mena22787@excite.com) Date: Wed Mar 5 11:59:48 2008 Subject: [Zbrafish] whole mount antibody staining of muscle Message-ID: <386025f9-f2fa-4d31-9837-38667b7ca880@u69g2000hse.googlegroups.com> I am an undergraduate student trying to stain skeletal muscle in embryos that are between 10-24 hpf. Thus far I've tried anti- sarcomeric actin, zm-1, and zm-3 antibodies using a protocol that is a modification of one found in the Zebrafish book. I have found either very dark staining of the outside/periphery of the embryo or dark staining of the yolk, but no specific muscle staining. We are using the Vectastain ABC detection kit. Does anyone have any recommendations or ideas on why we're getting this dark staining on the outside with no inside staining? (We are using the -20C acetone permeabilization step.) Serena Kankash- mena22787@excite.com From lionel.aubergat from laposte.net Mon Mar 10 02:33:34 2008 From: lionel.aubergat from laposte.net (lionel.aubergat@laposte.net) Date: Mon Mar 10 12:49:24 2008 Subject: [Zbrafish] In vitro Fertilization Message-ID: Hello, I'm a French Master student (of research) and I work in a laboratory of uranium toxicology. I'm working with zebrafish and I would like to fertilize eggs with an in vitro method. In the website "zfin.org" it was explained this fertilization. I have tried to do this fertilization but it did not work. When I gently squeeze female, I only get 4 eggs and for the male, I have never get sperm. So I have killed the fish and removed gonads. I have put them in a hank saline solution and I have dissociated testes with pestle. But when I have put the sperm on the eggs, I have added water to activate the sperm but I have never seen fertilization (after 1 hour). Can somebody know how to fertilize eggs with this method ? Best regards Lionel From karen.leewingman from gmail.com Tue Mar 11 03:14:33 2008 From: karen.leewingman from gmail.com (karen.leewingman@gmail.com) Date: Tue Mar 11 11:41:04 2008 Subject: [Zbrafish] Re: In vitro Fertilization References: Message-ID: <232ec6d5-68cc-4def-ad09-c8fc048209ef@q78g2000hsh.googlegroups.com> Salut, For the oocytes collection, they do not give oocytes everyday. They do have a cycle. So usually there are only a few female fish have good quality oocytes per day. Try to squeeze the female within an hour after the light comes on. The oocytes will be re-absorbed and there are no good for fertilization anymore. For the sperm, it is fairly easy to collect. When you squeeze the male, the whitish liquid is the sperm already. To check the motility of the sperm, you can put a drop (you collect the sperm and put them into the sperm extender solution) of the sperm onto the slide and use a 10X objective to have a look. It is not motile, but as soon as you add some water onto it, the sperm will start to swim. For more information, please check the following paper. Lee, K.W., Webb, S.E., and Miller, A.L. (1999). A wave of free cytosolic calcium traverses zebrafish eggs on activation. Developmental Biology 214: 168-180. If you need any further information, please feel free to contact me at karen.leewingman@gmail.com (Paris). From marta.manzoni from gmail.com Tue Mar 11 06:19:02 2008 From: marta.manzoni from gmail.com (martam) Date: Tue Mar 11 11:41:33 2008 Subject: [Zbrafish] Fluke-tabs and spawning Message-ID: Hi everybody, I used anti fluke tabs to remove trematodes from our zebrafish colony. I used the dosage recommended by the supplier, and it seems the treatment worked well. But, right now I have problems with zebrafish spawning and I am quite frustrated. Fishes seem to be healthy, but actually males do not look very "yellow" and apparently females do not get gravid. Has anyone ever observed anything similar? Does anyone have any suggestion to improve spawning (water parameters are ok..)? Thank you marta From shaukatali134 from gmail.com Tue Mar 11 11:37:02 2008 From: shaukatali134 from gmail.com (shaukat) Date: Tue Mar 11 11:41:56 2008 Subject: [Zbrafish] Re: buffer solution References: Message-ID: <68e39ebc-eaf0-481f-8f04-fa1d693e0fa9@n77g2000hse.googlegroups.com> On Mar 5, 9:46?am, fran?ois wrote: > On Mar 4, 7:57?pm, shaukat wrote: > > > I am determining ammonia toxicity on Zebrafish eggs and larvea.I am > > looking a non lethal buffer solution to maintain pH of ammonia > > solutions. > > Tris-HCl buffer works for me at 0.1M when dissolved in fish medium; > > fish medium: > Salts composition ? ? ? Concentration [mM] > CaCl2 * 2 H2O ? 2 > MgSO4 * 7 H2O ? 0.5 > NaHCO3 ? ? ? ? ? ? ? ? ?0.7 > KCl ? ? ? ? ? ? ? ? ? ? 0.07 > > hope it can help! > francois Dear Francois, Thank you very much for your help. Shaukat From shaukatali134 from gmail.com Tue Mar 11 11:40:36 2008 From: shaukatali134 from gmail.com (shaukat) Date: Tue Mar 11 11:42:15 2008 Subject: [Zbrafish] Ammonia toxicity Message-ID: Hi, I am looking for acute ammonia toxicity on different developmental stages of zebrafish.would you let m know please the protocol for this. Thanks Shaukat From clawrence from rics.bwh.harvard.edu Tue Mar 11 12:59:00 2008 From: clawrence from rics.bwh.harvard.edu (christian lawrence) Date: Tue Mar 11 13:01:33 2008 Subject: [Zbrafish] Fluke-tabs and spawning In-Reply-To: Message-ID: The symptoms you describe are generally associated with fish that are no longer in reproductive condition (assuming that they were previously). It probably isn't the fluke tabs themselves that have caused this unless you did not use them as directed or the brand you used is not appropriate for the zebrafish. Your problem (fish in non-reproductive condition) could be and is likely to be the result of a litany of things working in synergy, including: Advancing age Prolonged stress (and resource allocation towards immune function and away from everything else) Diet (bad diet -- most likely culprit) Genetics Poor "socializing" (leave the same reproductively active and healthy fish in the same tanks at densities below 5 fish per liter for a long time with no or little human intervention and watch what happens over the course of a month or two). Water quality (although you think it is fine, it may not be optimal) To make improvements, go from the simple forwards: 1. Clean up your fluke problem. This includes ensuring that you used the tabs properly and that they worked. 2. Ensure that all water quality parameters are favorable. 3. Ensure that your diet is complete and properly handled/administered. Formulated feeds should be no older than 3 months from process date, stored in sealed containers at 4C, and you should be feeding several different things (Artemia and pellet or flake). 4. Look at the age of the fish. Anything above 15 months is OLD and your chances of "restarting" them are lower the older they are past this age. This is particularly the case if you had a fluke infestation. This type of thing "ages" the fish and so a 15 month old fish that lived through a fluke epidemic is much "older" than one that didn't. 5. Improve social conditions. Mix and match fish from different tanks when possible. 6. Mate fish from disparate genetic backgrounds if possible. 7 (and 1a). Get some new fish from a reputable source or (even better) rear some yourself. Good luck, Chris Christian Lawrence Brigham and Women's Hospital Karp Family Research Laboratories 06-004B One Blackfan Circle Boston, Massachusetts 02115 Tel: 617.355.9041 Fax: 617.355.9064 -----Original Message----- From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of martam Sent: Tuesday, March 11, 2008 7:19 AM To: bionet-organisms-zebrafish@moderators.isc.org Subject: [Zbrafish] Fluke-tabs and spawning Hi everybody, I used anti fluke tabs to remove trematodes from our zebrafish colony. I used the dosage recommended by the supplier, and it seems the treatment worked well. But, right now I have problems with zebrafish spawning and I am quite frustrated. Fishes seem to be healthy, but actually males do not look very "yellow" and apparently females do not get gravid. Has anyone ever observed anything similar? Does anyone have any suggestion to improve spawning (water parameters are ok..)? Thank you marta _______________________________________________ Zbrafish mailing list Zbrafish@net.bio.net http://www.bio.net/biomail/listinfo/zbrafish The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From vammon from gmail.com Thu Mar 13 18:13:14 2008 From: vammon from gmail.com (virginia ammon) Date: Thu Mar 13 18:15:25 2008 Subject: [Zbrafish] transdermal peptide absorption in 2-4 dpf zf Message-ID: I have an assay i am trying to set up with 2-4 dpf zf where i want to add peptide to the water and i am concerned about the bio-availability. I am hoping that the peptide will be absorbed transdermally into the brain. As long as the peptide is soluble and small enough, does this even sound feasible? -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080313/2a7041b9/attachment.html From stefan.weigt from merck.de Fri Mar 14 08:22:33 2008 From: stefan.weigt from merck.de (stefan1306) Date: Fri Mar 14 12:06:37 2008 Subject: [Zbrafish] animal test Message-ID: Hi everybody, can anyone give me some information about the legal terms concerning the time (hours post fertilisation) when a zebrafish experiment is an in vitro test and when it is an animal test? I found only one citation: Braunbeck et al. in ALTEX 22, 2/05. Thanks Stefan From shaukatali134 from gmail.com Wed Mar 19 03:47:56 2008 From: shaukatali134 from gmail.com (shaukat) Date: Wed Mar 19 12:10:00 2008 Subject: [Zbrafish] Agarose Moulds Message-ID: <212927a2-66d0-4125-9920-72d91c5d502e@a23g2000hsc.googlegroups.com> Hi, does anybody know a company where we can buy the moulds for making agarose plates to put zebrafish embryos in so as to photograph them? They usually have 25 holes or wells in them. Thanks! Shaukat From shaukatali134 from gmail.com Wed Mar 19 03:51:43 2008 From: shaukatali134 from gmail.com (shaukat) Date: Wed Mar 19 12:11:30 2008 Subject: [Zbrafish] develpomental toxicity of different toxicins on zebrafish Message-ID: <5296c440-1cb2-4dac-ab51-824945f0d168@q78g2000hsh.googlegroups.com> Hi, Would any body let me know list of toxins according to the potentional toxicity. Thanks. Shaukat. Leiden university From nickjamescole from yahoo.com Wed Mar 19 17:59:05 2008 From: nickjamescole from yahoo.com (Nick Cole) Date: Wed Mar 19 18:18:28 2008 Subject: [Zbrafish] egg injector, fluorescent dissecting scope, wanted please Message-ID: <712437.33068.qm@web30104.mail.mud.yahoo.com> Hi There, I am setting up a zebrafish lab with very little startup money. Does anyone out there have an egg injector setup or a fluorescent dissecting scope that they are no longer using that you would be willing to part with? In fact any other zebrafish lab equipment would be greatly appreciated. Many thanks in advance, Nick nickjamescole@yahoo.com ___________________________________________________________ Rise to the challenge for Sport Relief with Yahoo! For Good http://uk.promotions.yahoo.com/forgood/ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080319/d16ef724/attachment.html From vishwasrao.paresh from gmail.com Thu Mar 20 06:18:13 2008 From: vishwasrao.paresh from gmail.com (vishwasrao.paresh@gmail.com) Date: Thu Mar 20 11:51:44 2008 Subject: [Zbrafish] Re: Abt Food of Zebrafish References: Message-ID: On Jan 23, 2:28 pm, prajakta dongre wrote: > Dear All Zebrafish Lovers > > I am student from Indian Institute of Science, > Bangalore. I am establishing zebrafish as a model in > Dr. Upendra Nongthomba's lab. > I have query regarding feeding of zebrafish with > Drosophila. > Should the fish be fed with etherized Drosophila? Will > etherized Drosophila affect Zebrafish Adults as well > as Fries? > Bye > Prajakta Limaye > > ____________________________________________________________________________________ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ Hi Prajakta, Feeding drosophila to zebrafish doesnt seem to be a good idea, Until unless you have specific question to be answered when other food is available. Though etherized fruitflies on my opinion shouldnt be having any problem. As dry food seems to work well as fish food. What are the questions you trying to answer in IIsc ? Would like to discuss in that area with respect to your time. with regards Paresh Vishwasrao Student pareshv22@gmail.com From cmoens from fhcrc.org Mon Mar 24 11:32:03 2008 From: cmoens from fhcrc.org (Cecilia Moens) Date: Mon Mar 24 12:12:05 2008 Subject: [Zbrafish] agarose molds (shaukat) In-Reply-To: <200803201703.m2KH3DL00163@net.bio.net> References: <200803201703.m2KH3DL00163@net.bio.net> Message-ID: <82D1BFB1-071D-4311-B11F-8AD10DDD44BB@fhcrc.org> Try Clive Ellard at Ellard Instrumentation clive@ellardinstrumentation.com Phone: (360) 805-5406 Fax: (360) 805-2877 14253 169th Dr. SE Suite 685 Monroe, Wa. 98272 He charges ~$40/mold. Remind him that you would like the injection molds he made for the Moens Lab at the Fred Hutch. He makes two types: one with a series of troughs, the other with holes for single embryos. Cecilia B. Moens Division of Basic Science Fred Hutchinson Cancer Research Center B2-152, 1100 Fairview Ave. N. Seattle, WA 98109-1024 office: (206) 667-5627 lab: (206) 667-5697 fax: (206) 667-3308 On Mar 20, 2008, at 10:03 AM, zbrafish-request@oat.bio.indiana.edu wrote: > Send Zbrafish mailing list submissions to > zbrafish@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/zbrafish > or, via email, send a message with subject or body 'help' to > zbrafish-request@net.bio.net > > You can reach the person managing the list at > zbrafish-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Zbrafish digest..." > > > Today's Topics: > > 1. Agarose Moulds (shaukat) > 2. develpomental toxicity of different toxicins on zebrafish > (shaukat) > 3. egg injector, fluorescent dissecting scope, wanted please > (Nick Cole) > 4. Re: Abt Food of Zebrafish (vishwasrao.paresh@gmail.com) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 19 Mar 2008 01:47:56 -0700 (PDT) > From: shaukat > Subject: [Zbrafish] Agarose Moulds > To: bionet-organisms-zebrafish@moderators.isc.org > Message-ID: > <212927a2-66d0-4125-9920-72d91c5d502e@a23g2000hsc.googlegroups.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, does anybody know a company where we can buy the moulds for making > agarose plates to put zebrafish embryos in so as to photograph them? > They usually have 25 holes or wells in them. Thanks! Shaukat > > > > ------------------------------ > > Message: 2 > Date: Wed, 19 Mar 2008 01:51:43 -0700 (PDT) > From: shaukat > Subject: [Zbrafish] develpomental toxicity of different toxicins on > zebrafish > To: bionet-organisms-zebrafish@moderators.isc.org > Message-ID: > <5296c440-1cb2-4dac-ab51-824945f0d168@q78g2000hsh.googlegroups.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > Would any body let me know list of toxins according to the potentional > toxicity. > > Thanks. > > > Shaukat. > > Leiden university > > > > ------------------------------ > > Message: 3 > Date: Wed, 19 Mar 2008 15:59:05 -0700 (PDT) > From: Nick Cole > Subject: [Zbrafish] egg injector, fluorescent dissecting scope, > wanted please > To: zbrafish@magpie.bio.indiana.edu > Message-ID: <712437.33068.qm@web30104.mail.mud.yahoo.com> > Content-Type: text/plain; charset="utf-8" > > Hi There, > > I am setting up a zebrafish lab with very little startup money. > > Does anyone out there have an egg injector setup or a fluorescent > dissecting scope that they are no longer using that you would be > willing to part with? In fact any other zebrafish lab equipment > would be greatly appreciated. > > Many thanks in advance, > > Nick > > nickjamescole@yahoo.com > > > > > ___________________________________________________________ > Rise to the challenge for Sport Relief with Yahoo! For Good > > http://uk.promotions.yahoo.com/forgood/ > -------------- next part -------------- > An HTML attachment was scrubbed... > URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080319/ > d16ef724/attachment-0001.html > > ------------------------------ > > Message: 4 > Date: Thu, 20 Mar 2008 04:18:13 -0700 (PDT) > From: vishwasrao.paresh@gmail.com > Subject: [Zbrafish] Re: Abt Food of Zebrafish > To: bionet-organisms-zebrafish@moderators.isc.org > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > On Jan 23, 2:28 pm, prajakta dongre wrote: >> Dear All Zebrafish Lovers >> >> I am student from Indian Institute of Science, >> Bangalore. I am establishing zebrafish as a model in >> Dr. Upendra Nongthomba's lab. >> I have query regarding feeding of zebrafish with >> Drosophila. >> Should the fish be fed with etherized Drosophila? Will >> etherized Drosophila affect Zebrafish Adults as well >> as Fries? >> Bye >> Prajakta Limaye >> >> >> _____________________________________________________________________ >> _______________ >> Be a better friend, newshound, and >> know-it-all with Yahoo! Mobile. Try it now. http:// >> mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > > Hi Prajakta, > Feeding drosophila to zebrafish doesnt seem to be a good idea, Until > unless you have specific question to be answered when other food is > available. > Though etherized fruitflies on my opinion shouldnt be having any > problem. As dry food seems to work well as fish food. > What are the questions you trying to answer in IIsc ? Would like to > discuss in that area with respect to your time. > > with regards > Paresh Vishwasrao > Student > pareshv22@gmail.com > > > > ------------------------------ > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > End of Zbrafish Digest, Vol 34, Issue 9 > *************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080324/64000a2a/attachment-0001.html