[Zbrafish] Re: Phenol Red

[Caroline Parkin]

Regarding phenol red, we use it direct (unmodified) from Sigma,catalog  
number P0290, at around 5-10% concentration in our injection mix.
Hope that helps,
Caroline


On 5 Nov 2008, at 17:03, zbrafish-request from oat.bio.indiana.edu wrote:

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>   1. Albino Zebrafish (FritzXC23)
>   2. about phenol red solution for microinjection (Clonfish)
>   3. RE: Albino Zebrafish (Burdine, Rebecca D)
>   4. TU wild type fish (Chao Zhang)
>   5. RE: TU wild type fish (David Lains)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 3 Nov 2008 19:40:25 -0800 (PST)
> From: FritzXC23 <fritsa01 from gettysburg.edu>
> Subject: [Zbrafish] Albino Zebrafish
> To: bionet-organisms-zebrafish from moderators.isc.org
> Message-ID:
> 	<568d4ada-66d3-4241-ae87-0ba50af9d99c from w39g2000prb.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi everyone! I am a junior undergraduate biochemistry major at
> Gettysburg College and I am working on a research project that
> investigates the effects of vitamin A deficiency on the development of
> ocular colobomas. I am trying to depigment the zebrafish embryos using
> phenylthiourea. I was wondering if anyone has used this tyrosinase
> inhibitor before, and if so at what time and for how long were the
> embryos dosed in a 0.003% solution of PTU? Thanks so much for your
> help!
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 4 Nov 2008 01:49:57 -0800 (PST)
> From: Clonfish <sunyonghua from gmail.com>
> Subject: [Zbrafish] about phenol red solution for microinjection
> To: bionet-organisms-zebrafish from moderators.isc.org
> Message-ID:
> 	<89f0e26f-4a1d-4739-84de-35cbcd9c43e5 from t18g2000prt.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
>
> I am trying to make a 5X stock phenol red solution for microinjection.
> When I searched the literature, I found different formulas from
> different papers, e.g., (0.1% phenol red, 100mM KCl) or (0.5% phenol
> red, 240 mM KCl) or (1% phenol red, 200mM KCl). Do you have any idea
> that which one gives the best result in your hands?
>
> Another question is that when I prepared 1.2% phenol red solution, it
> was really hard for the phenol red to be dissolved completely. Does
> anybody have any suggestion for that?
>
> Thanks a lot!
>
> Yonghua
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 4 Nov 2008 13:48:29 -0500
> From: "Burdine, Rebecca D" <rburdine from Princeton.EDU>
> Subject: RE: [Zbrafish] Albino Zebrafish
> To: "FritzXC23" <fritsa01 from gettysburg.edu>
> Cc: Zbrafish from magpie.bio.indiana.edu
> Message-ID:
> 	<04AF14C4EF3CF34682460FB1FDBA98C03F9701 from MBCLUSTER.pu.win.princeton.edu 
> >
> 	
> Content-Type: text/plain;	charset="us-ascii"
>
> Hi,
>
> If you want to inhibit most of the melanin, we put embryos into the  
> PTU
> solution during somitogenesis and then raise them in the solution from
> this point on.  If you take them out of the solution, melanin  
> synthesis
> can resume.
>
> Two key things we have found to be important when suing PTU: 1) We  
> make
> this fresh every two weeks or so and keep it in a bottle wrapped in
> foil. 2) You need to change the embryo media containing PTU everyday  
> to
> keep the melanin production down and the embryos happy and healthy.   
> (Of
> course you have to remove all the dying embryos and debris too as you
> would normally do.)
>
> Hope this helps,
> Becky
>
> ---------------------------------------------------
> Rebecca D. Burdine, Ph.D.
> Assistant Professor
> Dept. of Molecular Biology
> Princeton University
> Washington Road Mof 433
> Princeton, NJ 08544
>
> Phone: (609) 258-7515
> Fax: (609) 258-1343
> Email: rburdine from princeton.edu
> Admin Assistant: Cathy Falk (609) 258-1604
>
>> -----Original Message-----
>> From: zbrafish-bounces from oat.bio.indiana.edu [mailto:zbrafish-
>> bounces from oat.bio.indiana.edu] On Behalf Of FritzXC23
>> Sent: Monday, November 03, 2008 10:40 PM
>> To: bionet-organisms-zebrafish from moderators.isc.org
>> Subject: [Zbrafish] Albino Zebrafish
>>
>> Hi everyone! I am a junior undergraduate biochemistry major at
>> Gettysburg College and I am working on a research project that
>> investigates the effects of vitamin A deficiency on the development  
>> of
>> ocular colobomas. I am trying to depigment the zebrafish embryos  
>> using
>> phenylthiourea. I was wondering if anyone has used this tyrosinase
>> inhibitor before, and if so at what time and for how long were the
>> embryos dosed in a 0.003% solution of PTU? Thanks so much for your
>> help!
>>
>> _______________________________________________
>> Zbrafish mailing list
>> Zbrafish from net.bio.net
>> http://www.bio.net/biomail/listinfo/zbrafish
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 4 Nov 2008 12:16:15 -0800
> From: "Chao Zhang" <zhangch from ohsu.edu>
> Subject: [Zbrafish] TU wild type fish
> To: zbrafish from magpie.bio.indiana.edu
> Message-ID: <F58AB4839D379649A9E190A61925764A02040A from EX-BE06.ohsu.edu>
> Content-Type: text/plain; charset="gb2312"
>
> Is anybody using TU wild type fish?
> We need a little bit (10 embryos) for our research.
> Thanks.
> -------------- next part --------------
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> ------------------------------
>
> Message: 5
> Date: Tue, 4 Nov 2008 14:35:56 -0800
> From: "David Lains" <david from zebrafish.org>
> Subject: RE: [Zbrafish] TU wild type fish
> To: "'Chao Zhang'" <zhangch from ohsu.edu>,
> 	<zbrafish from magpie.bio.indiana.edu>
> Message-ID: <06df01c93ecd$b718e690$254ab3b0$@org>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Chao Zhang
>
>
>
> The Tu line is available as embryos from ZIRC.
>
>
>
> If you would like you can place an order at www.zebrafish.org.
>
>
>
> Please feel free to contact me if you have any questions or concerns.
>
>
>
> Best Fishes
> David Lains <}}}><
> Aquaculturist, Research Assistant
> Zebrafish International Resource Center
> 5274 University of Oregon
> Eugene, Or 97403
>
> Email:  <mailto:david from zfin.org> david from zebrafish.org
> pH: (541) 346-6028 ext. 18
> fax: (541) 346-6151
>
>
>
> From: zbrafish-bounces from oat.bio.indiana.edu
> [mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of Chao Zhang
> Sent: Tuesday, November 04, 2008 12:16 PM
> To: zbrafish from magpie.bio.indiana.edu
> Subject: [Zbrafish] TU wild type fish
>
>
>
> Is anybody using TU wild type fish?
> We need a little bit (10 embryos) for our research.
> Thanks.
>
> -------------- next part --------------
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