[Zbrafish] detecting 2 bp SSLP

[Thomas Bartman]

We're doing a positional cloning project and have an SSLP marker with 
a 2-bp polymorphism.  We can pick up the polymorphism with SSCP 
non-denaturing, cold acrylamide gels sometimes, but not reliably on 
even high percentage agarose gels.  Has anyone used denaturing 
acrylamide gels for this purpose, and do you have a protocol for the 
gel composition and sample prep before loading?  Would it be the same 
as what is used for sequencing gels?



Thomas Bartman, M.D., Ph.D.
Divisions of Neonatology, Pulmonary Biology, and Developmental Biology
Cincinnati Children's Hospital Medical Center
3333 Burnet Ave, MLC 7009
Cincinnati, OH  45229-3039

Office: 513-636-9902