From hmcallis from Princeton.EDU Tue Sep 2 09:41:23 2008 From: hmcallis from Princeton.EDU (Heather S McAllister (hmcallis@Princeton.EDU)) Date: Tue Sep 2 11:01:53 2008 Subject: [Zbrafish] Re: tank cleaning (Padnos.Beth@epamail.epa.gov) In-Reply-To: <200808301703.m7UH3GU17102@net.bio.net> References: <200808301703.m7UH3GU17102@net.bio.net> Message-ID: We use a barmaid to clean the polycarbonate tanks. Much easier than cleaning by hand. Here is the website: http://www.barmaidwashers.com/electric-glass-washers.aspx We use the A series, but I'm sure the SS series would work as well. We bought ours from www.webstaurantstore.com., catalog number 929A200 (the barmaid 1-200)model. Make sure you buy an extra 7.5" brush as the shorter ones don't work as well for cleaning. It isn't very helpful in cleaning AHAB 9 liters but it's sooooo much easier to clean the 1L and 2L. Heather McAllister Research Specialist II Burdine Lab Department of Molecular Biology Princeton University Princeton, NJ 08544 hmcallis@princeton.edu 609-258-5782 > > Message: 2 > Date: Fri, 29 Aug 2008 16:11:09 -0400 > From: Padnos.Beth@epamail.epa.gov > Subject: [Zbrafish] tank cleaning > To: zbrafish@oat.bio.indiana.edu > Message-ID: > 852574B4.006EE19C@epamail.epa.gov> > Content-Type: text/plain; charset=US-ASCII > > Does anyone have a good method for cleaning polycarbonate tanks? > Runningthe tanks through our cage washer does not get much, if > any, of the > debris off the surfaces. > > > > ------------------------------ > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > End of Zbrafish Digest, Vol 39, Issue 15 > **************************************** > From jocelyn.mcauley from gmail.com Wed Sep 3 13:02:12 2008 From: jocelyn.mcauley from gmail.com (Jocelyn) Date: Wed Sep 3 13:10:43 2008 Subject: [Zbrafish] Re: tank cleaning (Padnos.Beth@epamail.epa.gov) References: <200808301703.m7UH3GU17102@net.bio.net> Message-ID: <2d32965d-07fd-4a4e-9dd9-6bea454ea86e@a8g2000prf.googlegroups.com> On Sep 2, 7:41 am, "Heather S McAllister (hmcal...@Princeton.EDU)" wrote: > We use a barmaid to clean the polycarbonate tanks. Much easier than cleaning by hand. Here is the website:http://www.barmaidwashers.com/electric-glass-washers.aspx > We use the A series, but I'm sure the SS series would work as well. We bought ours fromwww.webstaurantstore.com., catalog number 929A200 (the barmaid 1-200)model. > Make sure you buy an extra 7.5" brush as the shorter ones don't work as well for cleaning. It isn't very helpful in cleaning AHAB 9 liters but it's sooooo much easier to clean the 1L and 2L. interesting suggestion! Heather- how is it installed in your fish facility? (in a sink, or a tub?) Do you run it submerged? thanks From hmcallis from Princeton.EDU Thu Sep 4 12:43:49 2008 From: hmcallis from Princeton.EDU (Heather S McAllister (hmcallis@Princeton.EDU)) Date: Thu Sep 4 13:07:22 2008 Subject: [Zbrafish] Re: tank cleaning (Padnos.Beth@epamail.epa.gov) (Jocelyn) Message-ID: It's portable, so we fill the sink with RO water when necessary and put the washer in and run it like that. We don't put anything into the water. If you have tanks that have dried out and have crusted algae, etc. it does a TERRIFIC job cleaning them. I doesn't scratch the plastic. We use it to clean the lids quickly and we rarely have food buildup on them anymore. It take about 20 seconds to clean one tank. If I am cleaning food-encrusted lids, I soak them for about 20 minutes in RO water and then run them through (about 4 seconds each lid) and they come out pristine. We only bleach tanks if they have come in contact with a dead or sick fish, in which case I don't use the barmaid. Hope this answers some questions, it has made my job sooooooooooooo much easier. If anyone has any more, feel free to ask me. Heather McAllister Research Specialist II Burdine Lab Department of Molecular Biology Princeton University Princeton, NJ 08544 hmcallis@princeton.edu 609-258-5782 From neil.a.campbell from gmail.com Fri Sep 5 10:18:08 2008 From: neil.a.campbell from gmail.com (Neil) Date: Fri Sep 5 12:35:02 2008 Subject: [Zbrafish] Re: tank cleaning (Padnos.Beth@epamail.epa.gov) (Jocelyn) References: Message-ID: <57fad576-47de-4269-ba6f-f13cf7c2c23a@f63g2000hsf.googlegroups.com> We have a barmaid here as well. I agree that it really does a good job on the smaller AHAB tanks and it saves me a load of time. Neil Campbell Sabaawy Lab The Cancer Institute of New Jersey Division of Medical Oncology, UMDNJ 195 Little Albany Street New Brunswick NJ 08903-2681 732-235-7148 From ksjones from westernu.edu Mon Sep 8 14:00:21 2008 From: ksjones from westernu.edu (westernfish) Date: Mon Sep 8 17:29:03 2008 Subject: [Zbrafish] Herpes Virus and Zebrafish Message-ID: Hi, I am trying to determine whether zebrafish larvae are succeptible to infection by herpesvirus. Does anyone have anexperience with this or can point me in the right direction? thanks! From zhangch from ohsu.edu Thu Sep 11 12:31:23 2008 From: zhangch from ohsu.edu (Chao Zhang) Date: Fri Sep 12 11:08:47 2008 Subject: [Zbrafish] Zebrafish TILLING Service Message-ID: Hi: Does anybody know some Zebrafish TILLING Services? I neede to create A specific gene KO founder and would like to pay for it. Thanks. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080911/16d6bdf7/attachment.html From zfinadmin from gmail.com Fri Sep 12 18:28:44 2008 From: zfinadmin from gmail.com (zfinadmin@gmail.com) Date: Fri Sep 12 18:29:42 2008 Subject: [Zbrafish] Antibody question Message-ID: Hi - does anyone know of antibodies that recognize products of zebrafish cdkn1b (p27,Kip1) or cdkn1c (p57,Kip2)? Thanks! From zhangch from ohsu.edu Wed Sep 17 11:56:11 2008 From: zhangch from ohsu.edu (Chao Zhang) Date: Wed Sep 17 15:03:32 2008 Subject: [Zbrafish] Pigmentary Mutates References: <200809121703.m8CH3KV17791@net.bio.net> Message-ID: Hi, there: Does anybody know where I can find genomic DNA or living fish of these two pigmentary mutates: fata morgana (famte267) submarine (sumtr6) I have tried Zfin already.No luck. Thanks. -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: application/ms-tnef Size: 2455 bytes Desc: not available Url : http://www.bio.net/bionet/mm/zbrafish/attachments/20080917/bddde79e/attachment.bin From daph from temple.edu Thu Sep 18 16:31:40 2008 From: daph from temple.edu (Daphne Georlette) Date: Thu Sep 18 17:34:50 2008 Subject: [Zbrafish] embryo nuclear extract Message-ID: <3B108142-7B00-438F-A3E9-95BD4D26EBDB@temple.edu> Hi everyone I'm new in the zebrafish field and I'm trying to find a protocol to make nuclear extracts from zebrafish embryos. I was thinking of using the low salt/high salt extraction method, but was wondering if any of you had a protocol working nicely! Thanks! Daphne ------------------------------------------------------- Daphne Georlette, Ph.D. Research Assistant Professor Biemar & Georlette Lab Department of Biology Temple University 436 Biology Life Sciences Building 1900 North 12th Street Philadelphia, PA 19122, USA Tel. 1-215-204-0291 Fax. 1-215-204-6646 http://openwetware.org/wiki/Biemar From alzaidan.abdullah from gmail.com Sun Sep 21 22:31:53 2008 From: alzaidan.abdullah from gmail.com (alzaidan.abdullah@gmail.com) Date: Mon Sep 22 12:20:46 2008 Subject: [Zbrafish] Re: ZebraFish Skin Histology References: Message-ID: <078e3dd3-8de8-4f0d-9f5f-80cc2ba38f1b@z6g2000pre.googlegroups.com> On Jul 26, 1:48 am, Katy wrote: > On Jul 21, 6:50 pm, alzaidan.abdul...@gmail.com wrote: > > > Hi, > > >  I’m currently studying the effect of contaminants on the skin of > > Zebrafish through Histology. The problem I’m facing while processing > > the tissue is after doing so the skin always detaches from the muscle. > > The fixative used is 4% NBF for 24 hrs. And I’m only removing the skin > > attached with muscle, also tried to cut a TS section (Dorsal to > > Ventral through the vertebra) but similar effect. The thickness used > > on microtome is 5um. > > > Any help would be appreciated > > > Abdullah Al-Zaidan > > Hello, > Are you looking at H&E stained sections?  At the ZIRC we use > Dietrich's fixative for all H&E specimens.  I've pasted our fixation > protocol with a recipe for Dietrich's fixative  below.  Hope this > helps. > Katy Murray, DVM, Ph.D. > Zebrafish International Resource Center > >     *    Fixation Protocol for Histopathology Specimens >     * Prepare Dietrich's Fixative (100 ml) as follows. Store fixative > at room temperature. >            30 ml Ethanol (95%) >            10 ml Formalin (Formaldehyde 37% solution, histological > grade, contains 10-15% methanol, Sigma # F1635) >            2 ml Glacial Acetic Acid >            58 ml Distilled Water >     * Euthanatize fish with an overdose of MS-222 (tricaine methane > sulfonate). >     * Carefully slit open the body cavity along the belly, trying not > to disrupt internal organs. >     * Use 10-15 ml of fixative per 1-2 fish in a sealable container. > The volume of fixative should be at least 10 times the volume of the > specimen. >     * Store and ship fixed fish in Dietrich's fixative. Fish can be > stored for several weeks in Dietrich's fixative. Dear Katy: Thank you for your help i appreciate it. From smitra from abdn.ac.uk Mon Sep 22 10:39:09 2008 From: smitra from abdn.ac.uk (Mitra, Suman) Date: Mon Sep 22 12:21:12 2008 Subject: [Zbrafish] Inquiry Message-ID: Hello there1 This is Suman from King's college , Aberdeen. Recently I joined a new group working on zebrafish here in Aberdeen. I would like to join the zebrafish forum to get some information. Would you please advice me how to do that? Many thanks, Suman The University of Aberdeen is a charity registered in Scotland, No SC013683. From gothula74 from gmail.com Tue Sep 23 15:30:21 2008 From: gothula74 from gmail.com (gothula74@gmail.com) Date: Tue Sep 23 15:32:51 2008 Subject: [Zbrafish] Zebrafish-BLOOD DIAGNOSTICS, PCV and RBC count, etc. Message-ID: <447de918-272d-4e3e-a409-3f5faee450c5@a3g2000prm.googlegroups.com> Hello - Does anyone happen to have normal values for MCV and Hb for Zebrafish? Also, I have been asked to obtain the MCV and Hb levels for a specific line of Zebrafish. Is there a way to run PCV/Hct and do RBC counts for Zebrafish blood? I am thinking that I will not be provided with enough blood to run either; I was told the lab can obtain ~10uL from an adult fish. I'm thinking I need at least 80uL to run PCV and do a RBC count. If there is anyone out there that can give me some advice on this, I would greatly appreciate it. Thank you! From sspinette from ric.edu Wed Sep 24 13:37:09 2008 From: sspinette from ric.edu (Dr. S) Date: Wed Sep 24 13:40:27 2008 Subject: [Zbrafish] problems with spawning Message-ID: <7da47250-602b-4101-902e-678fc17f8a89@k30g2000hse.googlegroups.com> When we first set up our system over 2 years ago, we had a very sucessfull year and a half, no disease, lots of great large healthy collections. then suddenly things changed, fish got sick and stopped spawning. We tried isolation, water chages etc. finally we gutted everything and started fresh. A coupl of months ago we started up 2 new fresh 20 gal tanks (Biowheel). One for the males and one for the females. We had switched to "conditioned" MilliQ water containing bicarbonate, instant ocean and calcium sulphate such that our pH is between 7.2-7.4, our conductivity is 400-450microS, our ammonia is o nitrite is 0 nitrate is sometimes slighly measurable before we do the water change, temp is 27-28. we monitor these things every day. We do 10% water changes daily and clean the tank very well every month, replacing 50% of the water and the carbon filters. We feed the fish 2-3x per day artemia and dry food from Aquatic Ecosystems that they claim is the best formula. The fish look healthy although the males don't seem to eat as much as the females. The females bellies are large but they are not spawning at all. What else can we do? Please help. Thank You From dadams from forsyth.org Thu Sep 25 08:39:12 2008 From: dadams from forsyth.org (DSA) Date: Thu Sep 25 13:46:09 2008 Subject: [Zbrafish] Re: problems with spawning References: Message-ID: <397feda0-2714-487d-822d-de488c2b8d5c@r15g2000prd.googlegroups.com> On Sep 24, 2:37?pm, "Dr. S" wrote: > When we first set up our system over 2 years ago, we had a very > sucessfull year and a half, no disease, lots of great large healthy > collections. then suddenly things changed, fish got sick and stopped > spawning. We tried isolation, water chages etc. finally we gutted > everything and started fresh. > > A coupl of months ago we started up 2 new fresh 20 gal tanks > (Biowheel). One for the males and one for the females. We had switched > to "conditioned" MilliQ water containing bicarbonate, instant ocean > and calcium sulphate such that our pH is between 7.2-7.4, our > conductivity is 400-450microS, our ammonia is o nitrite is 0 nitrate > is sometimes slighly measurable before we do the water change, temp is > 27-28. we monitor these things every day. We do 10% water changes > daily and clean the tank very well every month, replacing 50% of the > water and the carbon filters. We feed the fish 2-3x per day artemia > and dry food from Aquatic Ecosystems that they claim is the best > formula. The fish look healthy although the males don't seem to eat as > much as the females. The females bellies are large but they are not > spawning at all. What else can we do? Please help. > > Thank You I've been through this, it's really frustrating. Once, it turned out the light timers were not working, which I hadn't noticed because the lights were always on during the day. It turned out they were on all night too. Since you've listed everything else that it turned out to be at one time or another, who knows. From jpilger from agnesscott.edu Wed Sep 24 15:32:58 2008 From: jpilger from agnesscott.edu (Pilger, John) Date: Thu Sep 25 13:46:28 2008 Subject: [Zbrafish] Apoptosis Message-ID: Does anyone have experience using Hoechst 33342 and propidium iodide (e.g. Invitrogen kit #5, cat. V13244) as an apoptosis assay for zebrafish? Specifically we want to look at apoptosis during lens formation. Any protocols or even concentration recommendations are welcome. John John F. Pilger, Ph.D. William Rand Kenan Jr. Professor Department of Biology Agnes Scott College 141 E. College Ave. Decatur, GA 30030-3770 voice: 404-471-6269 fax: 404-471-5368 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080924/62ead933/attachment.html From els.janssens from gmail.com Sat Sep 27 12:49:05 2008 From: els.janssens from gmail.com (Els) Date: Mon Sep 29 11:06:24 2008 Subject: [Zbrafish] Sense probes ISH Message-ID: Dear all, I'm having difficulties to make sense probes for whole mount in situ hybridization. There are no big differences in staining patterns between my sense and antisense probes. Is anyone familiar with the problem? What can I do to prevent this? Greetz, Els Janssens Els Janssens PhD-student Research Group Neural Circuit Development and Regeneration Department of Biology, Katholieke Universiteit Leuven (K.U.Leuven) Naamsestraat 61, bus 2464 B-3000 Leuven, Belgium Phone: +32 16 323987 Fax: +32 16 324262 E-mail: els.janssens@bio.kuleuven.be http://bio.kuleuven.be/df/LM/ From reiser.17 from osu.edu Mon Sep 29 11:51:01 2008 From: reiser.17 from osu.edu (Peter Reiser) Date: Mon Sep 29 12:03:58 2008 Subject: [Zbrafish] tamoxifen-induced gene expression in zfish? Message-ID: <2096B6FC591D034FA50AFFD7A42EE96505452F1D@dental.dentnet.dent.ohio-state.edu> Dear Gilbert, I came across your inquiry, at http://www.bio.net/bionet/mm/zbrafish/2006-April/005838.html, on possible toxicity of tamoxifen, and am interested in whether you received any useful information on its toxicity. I am reviewing a protocol for our IACUC and am seeking similar information. I would greatly appreciate a reply if you have any relevant information. With regards, Peter Peter J. Reiser, Ph.D. Professor, Oral Biology Ohio State University 4165 Postle Hall 305 West 12th Avenue Columbus OH 43210-1267 Office: 614-292-8476 Lab: 614-247-7335 Fax: 614-247-6945