[Zbrafish] Re: Construct/transposase [ ]

[haytcb from gmail.com]

well~This concentration should work well~~but I just wonder what you
mean by cRNA...

Actually, you should inject your constructs together with the
transposase mRNA.

Good luck :)

On Jan 27, 10:56 pm, Jonathan Schumacher <jaschu... from gmail.com> wrote:
> Greetings,
>
> I am a rookie with zebrafish transgenics and have been given a project
> to inject embryos with a construct in conjunction with transposase
> cRNA.  I don't see in the literature where the concentration of
> construct & transposase are described.  I've been using 20ng/uL for
> both construct & transposase.  Does this seem like a reasonable
> concentration?  I saw on another posting that Dr. Lister described
> using ng/uL concntrations.
> Thoughts?
>
> Many thanks!
>
> Jonathan Schumacher
> Research and Development Scientist
> Anatomic Pathology Molecular Diagnostics
> ARUP Institute for Clinical & Experimental Pathology
> 500 Chipeta Way
> Salt Lake City, UT 84108
> Telephone: 801-583-2787 extension 2958
> Fax: 801-584-5109
> Email: schu... from aruplab.com