[Zbrafish] number of cells of ZF 3-5d old embryos

[MILKA]

Hello,

Would anyone have an idea of how many total cells can be extracted
from 3,4 or 5 d old ZF embryos with trypsin/EDTA-mediated
dissociation? I am trying to prepare cell suspensions from ZF embryos
and would like to have an idea of how many cells/embryo are possible
to extract at the different developmental stages. Any insight would be
very much appreciated!

Many thanks,

Milka