[Zbrafish] embryo/larval survival

[Jim D.]

Hello.  I am planning a high throughput chemical screen on embryos/
larvae.  I'd like to do either a 96 well or 24 well format.  My
question:  does any one have a feel for how long an embryo can survive
after dechorionation in a 96 or 24 well plate?  If people have tried
this, have they modified the plates at all (for example, poking holes
in the lid).
Thanks!

Jim Dowling
Assistant Professor, Department of Pediatrics and Neurology
University of Michigan Medical Center