[Zbrafish] Re: embryo/larval survival

[padnos from yahoo.com]

On Feb 14, 2:06 pm, "Jim D." <jimdowlingmd... from mac.com> wrote:
> Hello.  I am planning a high throughput chemical screen on embryos/
> larvae.  I'd like to do either a 96 well or 24 well format.  My
> question:  does any one have a feel for how long an embryo can survive
> after dechorionation in a 96 or 24 well plate?  If people have tried
> this, have they modified the plates at all (for example, poking holes
> in the lid).
> Thanks!
>
> Jim Dowling
> Assistant Professor, Department of Pediatrics and Neurology
> University of Michigan Medical Center

I raise fry daily from about 6 hpf to 6 dpf in 96 well plates
(Millipore 96 well mesh plates which are now a special order) 1 per
well and have about 95 to 100 % survival.periodically have riased in
24 and 48 well plates without any trouble to the same age with the
same approximate result. We do not dechorinate.

Beth Padnos
US EPA RTP North Carolina