[Zbrafish] Zfish injection/integration control

[Jonathan Schumacher]

Greetings,

I have designed a construct of MITFA driving my oncogene and
cytoplasmic-expressed EGFP, subsequently cloned into the pDest
Tol2pA.  We suspect that it will take at least 8 weeks to see whether
the fish develop cancer and after setting up injections, I realized
that I have no injection/integration control.  My question is would
you suggest recloning my construct into the pDest Tol2pA with cmlc2-
driven EGFP?  I believe that this would lead to double-EGFP labeling
and am unclear if this would be problematic.  Is there an alternative
strategy, like coinjecting my destination vector with a cherry-labeled
cmlc2 (or other)?

Your thoughts & suggestions are greatly appreciated.

Best,

Jonathan