[Zbrafish] RALDH Protein Assay

[FritzXC23]

Hi! My name is Sarah and I am undergraduate Biochemistry student at
Gettysburg College. I am currently conducting an independent research
experiment investigating the effects of vitamin A deficiency on eye
development in zebrafish. To make the embryos vitamin A deficient I am
dosing them with various concentrations of disulphiram and DEAB (two
retinaldehyde dehydrogenase inhibitors). I was wondering if anyone
could give me advice on a protein assay that I could potentially
perform that would allow me to measure RALDH activity levels in the
dosed fish. I found an article that described a BioRad assay to
measure protein concentration followed by a simple spectrophotometric
assay at 350nm. The tissue preparation described for these two assays
in regards to RALDH activity, however, was from a human source. Has
anyone performed or heard of these assays and know how to prepare
zebrafish embryos for them? Or does anyone have a better suggestion
for an assay that I could reasonably perform? Thanks for your help!

Sarah