[Zbrafish] Re: Defeating the swimbladder

[Wilson Clements]

Dear Fish Gurus,

For some of our fluorescent transgenic lines, it would be useful to be  
able to easily screen individuals at, for example, 5 dpf.   
Unfortunately, this process is inhibited by variable swimbladder  
inflation.  I can tricaine my fish, and many of them remain on the  
bottom of a petri dish where I can easily observe and sort them.   
However a certain number of individuals have inflated swimbladders  
causing them to float out of microscopic focus and move around in the  
media.

I was wondering if anyone had ever come up with a trick to cause  
temporary swimbladder deflation in a plate.

Best,
Wilson Clements
--------------------------------------------------------------------------------------------
Wilson Clements, Ph.D.

wclements from ucsd.edu

Dept. of Biology
Section of Cell and Developmental Biology
University of California at San Diego
9500 Gilman Dr.
Natural Sciences Building 6105
La Jolla, CA 92093-0380

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