[Zbrafish] Re: Defeating the swimbladder

[Jim Burris]

Wilson,

When possible we sort nearly all of our fluorescent transgenic fish  
at 3 dpf to avoid this problem.

Best Fishes,
Jim Burris
Zebrafish Facility Manager
Department of Cell Biology
Duke University Medical Center
Durham, NC  27710
(919)684-6092
(843)344-1887 (cell)
j.burris from cellbio.duke.edu





On Jun 18, 2009, at 1:04 PM, zbrafish-request from oat.bio.indiana.edu wrote:

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>    1. Fish/trout serum for zebrafish cell culture (Lasse)
>    2. Re: Defeating the swimbladder (Wilson Clements)
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> Message: 1
> Date: Wed, 17 Jun 2009 10:21:22 -0700 (PDT)
> From: Lasse <lasse from scienceshow.dk>
> Subject: [Zbrafish] Fish/trout serum for zebrafish cell culture
> To: bionet-organisms-zebrafish from moderators.isc.org
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> Dear All
>
> A collegue and I are trying to establish fish cell lines, and in
> preparation for that I have found several papers strongly promoting
> the addition of Fish/trout/salmonoid serum and/or embryo extracts to
> the culture medium. I was wandering if anyone have experience with
> using fish serum for cell culture and if so, have a tip to where I can
> purchase fish serum relatively cheaply (preferably a european
> company). Alternative if you have a good protochol for how to obtain
> cell culture-grade fish serum from whole blood?
>
> I would also appreciate any other comments or expertise/know how you
> might like to share regarding fish cell culture, although I can be
> more specific at this time as I have just started myself.
>
> All my best, and thanks alot in advance!! Lasse
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 17 Jun 2009 13:10:10 -0700
> From: Wilson Clements <wclements from ucsd.edu>
> Subject: [Zbrafish] Re: Defeating the swimbladder
> To: zbrafish from magpie.bio.indiana.edu
> Message-ID: <2CD233FF-6365-41D6-89B1-B71EDC6037B4 from ucsd.edu>
> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
>
> Dear Fish Gurus,
>
> For some of our fluorescent transgenic lines, it would be useful to be
> able to easily screen individuals at, for example, 5 dpf.
> Unfortunately, this process is inhibited by variable swimbladder
> inflation.  I can tricaine my fish, and many of them remain on the
> bottom of a petri dish where I can easily observe and sort them.
> However a certain number of individuals have inflated swimbladders
> causing them to float out of microscopic focus and move around in the
> media.
>
> I was wondering if anyone had ever come up with a trick to cause
> temporary swimbladder deflation in a plate.
>
> Best,
> Wilson Clements
> ---------------------------------------------------------------------- 
> ----------------------
> Wilson Clements, Ph.D.
>
> wclements from ucsd.edu
>
> Dept. of Biology
> Section of Cell and Developmental Biology
> University of California at San Diego
> 9500 Gilman Dr.
> Natural Sciences Building 6105
> La Jolla, CA 92093-0380
>
> TEL    (858) 534-6955
> LAB    (858) 822-4658
> FAX    (858) 822-5740
>
>
>
>
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> End of Zbrafish Digest, Vol 49, Issue 8
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