[Zbrafish] Problems with DNAse

[Usua]

Dear all,

I am working with zebrafish embryos and gene expression. I use trizol
for the RNA extraction, and then when I use DNAse before the RT PCR, I
lose all the RNA in my sample.

does anyone use a DNAse step in their RNA preps?

I would like to know if it is neccessary to use dnases or if I could
play directly the PCR.  I know that my preps theoretically should not
yield DNA product, but i want to be sure that the RTPCR products I am
recieving are from an RNA template and not from DNA.

Thank you in advance
.