Southern.
-Louis
On Wed, 15 May 1996, Zhongguo Xiong wrote:
> Date: Wed, 15 May 1996 17:38:15 GMT
> From: Zhongguo Xiong <zxiong at ag.arizona.edu>
> To: methods at net.bio.net> Subject: Is this called Southern hybridization or Northern hybridization?
>> This may be a FAQ, but I have a hard time finding the answer.
>> I have performed hybridization using RNA probes (riboprobes) to
> hybridized DNA immobilized on nylone membrane. What is the correct
> terminology for this hybridization:
>> a). Southern hybridization.
> b). Northern hybridization.
> c). neither.
>> Since the DNA is blotted to a piece of membrane, it's reasonable to
> call it Southern hybridization. But the hybridization condition I used
> is for Northern hybridization (to maximize DNA:RNA duplex during
> hybridization). So it might as well be called Northern hybridization.
> Can anybody enlighten me on the history of these terms and how it is
> exactly defined?
>> Thanks,
>>> Xiong
>zxiong at ag.arizona.edu>>>>
Dr. Louis H. Ferland
Centre de Recherche, Hotel-Dieu de Montreal
Dept de Nutrition, Universite de Montreal
Phone: (514) 843-2757 FAX: (514) 843-2719
