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Transfection of mammalian cells

Jerome Ranch ranchjp at home.com
Sat Jun 10 10:29:11 EST 2000


I found this one


Szybalska and Szybalski  1962  Genetics of Human Cell Lines, IV.
DNA-mediated heritable transformation of a biochemical trait  PNAS
48:2026-2034

Used HAT selection to correct a IMPPase-deficient human cell line
(D98) by treating them with DNA derived from the progenitor non-mutant
cell line D98S.

Jerry



BTW  the review by
Felgner, P.L. Particulate systems and
polymers for in vitro and in vivo delivery of polynucleotides.  Adv.
Drug Deliv.  Rev.  5, 163–187 (1990)) is pretty good






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