I maintain the microbiology lab for a small college. This week I made a
modified Snyder Test Agar and after two days of incubation we have not
seen much change of color. I didn"t have the dye that was in the Difco
recipe so I substituted Brom-Phenol Blue. The list of pH indicators
showed it as changing color at a pH comparable to the recommended dye.
Can anyone tell me if my substitution should work? The students will be
checking the tubes again on Tuesday. Also, I have been buying
sheep's blood agar plates for the classes but I would like to start
pouring them myself. Is there anything special I should know about this
or is it just another media to make?